Zhang lianwen
Associate Professor
· Work Address: College of Pharmacy, Nankai University
· Telephone: +86-22-***
· E-mail: lianwen@nankai.edu.cn
Research Areas
· Glycobiology：We mainly focuse on the study of protein O-GlcNAcylation (an important post-translational modification), including the enzymes (OGT & OGA) orchestrating the modification, the O-GlcNAc role on specific proteins, O-GlcNAc variation in ageing process and the signal transduction pathways.
· Biomarkers. We are interested in the examination of O-GlcNAc proteins in various clinical samples (tissue/blood/urine) to find valuable biomarkers.
· Cross-talks between O-GlcNAcylation and ubiquitination.
Research & Achievements
Peer-Reviewed Journal Papers: (*Corresponding author)
[1]. Ningning Tang#, Ling Li1#, Fei Xie#, Ying Lu, Zifan Zuo, Hao Shan, Quan Zhang* and Lianwen Zhang*, A living cell-based fluorescent reporter for high-throughput screening of anti-tumor drugs, J. Pharm. Anal., 2021, DOI: https://doi.org/10.1016/j.jpha.2021.04.001 (JPHA 645).
[2]. Li Liu#, Ling Li#, Cheng Ma#, Yangde Shi , Congcong Liu, Zikang Xiao, Yong Zhang, Fang Tian, Yang Gao, Jie Zhang, Wantao Ying*, Peng George Wang & Lianwen Zhang* O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity, Journal of biological chemistry, 2019, 294(45): 16620-16633.
[3]. Chuanhui Zhang#, Fei Xie#, Ling Li#, Cheng Zhang, Yong Zhang, Wantao Ying, Li Liu, Xuli Yan, Futao Yin and Lianwen Zhang*, Hepatocyte nuclear factor 1 alpha (HNF1A) regulates transcription of O-GlcNAc transferase in a negative feedback mechanism, FEBS letters, 2019,593:1050-1060.
[4]. Kuan Jiang, Lei Li, He Zhu, Yuxi Guo, Ebtesam Gashash, Cheng Ma, Xiaolin sun, Lianwen Zhang*, Peng George Wang*, Sialic acid linkage-specific permethylation for isomeric and sensitive profiling of sialylated glycans by MALDI-TOF MS，Analytica Chimica Acta，2017 (981): 53-61. (co-corresponding author).
[5]. Kuan Jiang, Arya Aloor, Jiangyao Qu, Cong Xiao, Zhigang Wu, Cheng Ma, Lianwen Zhang*, Peng George Wang *, Rapid and sensitive MALDI MS analysis of oligosaccharide by using 2-hydrazinopyrimidine as a derivative reagent and co-matrix, Anal Bioanal Chem, 2017, 409(2): 421-429. (co-corresponding author).
[6]. Jiang, K., B. Bai, Y. Ta, T. Zhang, Z. Xiao, P. G. Wang* and L. Zhang* O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway. Biochem Biophys Res Commun. 2016, 471(4):539-44.
[7]. Wu, Zhigang; Jiang, Kuan; Zhu, Hailiang; Ma, Cheng; Yu, Zaikuan; Li, Lei; Guan, Wanyi; Liu, Yunpeng; Zhu, He; Chen, Yanyi; Li, Shanshan; LI, Jing; Cheng, Jiansong; Zhang, Lianwen* and Wang, Peng*, Site-directed glycosylation of peptide/protein with homogeneous O-linked eukaryotic N-glycans, Bioconjugate Chemistry, 2016, 27 (9): 1972–1975. (co-corresponding author)
[8]. Jiang, K., Y. Gao, W. Hou, F. Tian, W. Ying, L. Li, B. Bai, G. Hou*, P. G. Wang* and L. Zhang* Proteomic analysis of O-GlcNAcylated proteins in invasive ductal breast carcinomas with and without lymph node metastasis. Amino Acids 2016, 48(2): 365-374.
[9]. Han, X., L. Qian, L. Zhang* and X. Liu* Structural and biochemical insights into nucleotide-rhamnose synthase/epimerase-reductase from Arabidopsis thaliana. Biochim Biophys Acta, 2015, 1854(10 Pt A): 1476-1486. (co-corresponding author)
[10]. Xiaoyan Liu, Ling Li, Yuqiu Wang, Hui Yan, Xiaofeng Ma, Peng George Wang, Lianwen Zhang*, A peptide panel investigation reveals the acceptor specificity of O-GlcNAc Transferase, FASEB J. 2014, 28(8): 3362-3372.
[11]. Zhou, F., H. Yan, L. Li, X. Y. Liu, Y. Q. Wang, P. Wang* and L. W. Zhang* FBXW10 is negatively regulated in transcription and expression level by protein O-GlcNAcylation. Biochem Biophys Res Commun. 2013,438(2): 427-432.
[12]. Xiaofeng Ma, Pi Liu, Hui Yan, Hong Sun, Xiaoyan Liu, Feng Zhou, Lei Li, Yi Chen, Musleh M. Muthana, Xi Chen, Peng george Wang* and Lianwen Zhang*. Substrate specificity provides insights into the molecular basis of O-GlcNAc transferase (OGT) – sugar donor recognition. PLoS ONE. 2013, 8 (5): e63452.
[13]. Guangxiang Ren, Jingli Hou, Qinghong Fang, Hong Sun, Peng George Wang and Lianwen Zhang* Synthesis of flavonol 3-O-glycoside by UGT78D1, Glycoconjugate Journal, 2012, 29 (5):425-432
Teaching
[1] Undergraduate: Glycobiology and diseases
[2] Master: Molecular biotechnology and bioseparation
